Polyoxypregnane Glycosides from Root of Marsdenia tenacissima and Inhibited Nitric Oxide Levels in LPS Stimulated RAW 264.7 Cells.

Six new polyoxypregnane glycosides, marstenacisside F1-F3 (-), G1-G2 (-) and H1 (), as well as 3--β-D-glucopyranosyl-(1→4)-6-deoxy-3--methyl-β-D-allopyranosyl-(1→4)-β-D-oleandropyranosyl-11α,12β-di--benzoyl-tenacigenin B (), were isolated from the roots of . Their structures were established by an extensive interpretation of their 1D and 2D NMR and HRESIMS data. Compounds - were tenacigenin B derivatives with an oligosaccharide chain at C-3. This was the first time that compound had been isolated from the title plant and its H and C NMR data were reported. Compounds and were the first examples of C steroid glycoside bearing unique β-glucopyranosyl-(1→4)-β-glucopyranose sugar moiety. All the isolated compounds were evaluated for anti-inflammatory activity by inhibiting nitric oxide (NO) production in the lipopolysaccharide-induced RAW 264.7 cells. The results showed that marstenacisside F1 and F2 exhibited significant NO inhibitory activity with an inhibition rate of 48.19 ± 4.14% and 70.33 ± 5.39%, respectively, at 40 μM, approximately equal to the positive control (L-NMMA, 68.03 ± 0.72%).