Bamboo charcoal mediated plant secondary metabolites biosynthesis in tomato against South American tomato pinworm (Tuta absoluta)

IntroductionThe south American tomato pinworm (Tuta absoluta), an invasive insect pest species, has established itself in more than 33 Chinese prefectures, where it is mainly damaging tomato crops. Immediate efforts have been initiated to find strategies to control this pest. Studies have shown that biochar (BC) amendment to soil can enhance plant growth and resistance to herbivory. MethodsFirst, we quantified the morphological performance of tomato plants grown in different coconut bran and bamboo charcoal (v/v) combinations and selected the most beneficial one. Then we checked the T. absoluta survival on the tomato plants grown in the selected bamboo charcoal combination. Finally, we studied the leaf metabolite accumulation and gene expression changes in tomato plants after growing in the selected bamboo charcoal combination. ResultsWe found that the 30:1 ratio of bamboo charcoal and coconut bran is the most beneficial to tomato growth as its amendment to soil increased tomato plant height, stem thickness, and chlorophyll content, whereas, the T. absoluta survival decreased. The metabolome profiles of BC tomato leaves showed an increased accumulation of flavonoids, terpenoids, and phenolic acids compared to CK. Transcriptome sequencing resulted in the identification of 244 differentially expressed genes. Most of the upregulated genes were associated with stress-related hub proteins, flavonoid biosynthesis, MAPK and phytohormone signaling, and terpenoid biosynthesis. Additionally, the expression of many genes related to signaling and defense was changed in response to the bamboo charcoal amendment. DiscussionWe conclude that bamboo charcoal induces biosynthesis of flavonoids, terpenoids, and phenolic acids, which improve plant growth and tolerance against T. absoluta, thus reducing the survival of destructive pests.