Ralstonia wenshanensis sp. nov., a novel bacterium isolated from a tobacco field in Yunnan, China.

A Gram-negative, aerobic, motile with paired polar flagella and rod-shaped bacterium strain (56D2) was isolated from tobacco planting soil in Yunnan, PR China. Major fatty acids were C 7 (summed feature 3), C and C  7 (summed feature 8). The polar lipid profile of strain 56D2 consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid and one unidentified glycolipid. Moreover, strain 56D2 contained ubiquinone Q-8 as the sole respiratory quinone. 16S rRNA gene sequence analysis showed that strain 56D2 was closely related to members of the genus and the two type strains with the highest sequence identities were LMG 6866 (98.36 %) and K-288 (98.22 %). The 16S rRNA gene sequence identities between strain 56D2 and other members of the genus were below 98.00 %. Genome sequencing revealed a genome size of 5.87 Mb and a G+C content of 63.7 mol%. The average nucleotide identity values between strain 56D2 and K-288, LMG 6866 and CCUG 46789 were less than 95 %, and the DNA-DNA hybridization values (yielded by formula 2) were less than 70 %. Based on these data, we conclude that strain 56D2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of sp. nov. is 56D2 (=CCTCC AB 2021466=GDMCC 1.2886=JCM 35178).