Planarian LDB and SSDP proteins scaffold transcriptional complexes for regeneration and patterning
bioRxiv (Cold Spring Harbor Laboratory)(2023)
摘要
Sequence-specific transcription factors often function as components of large regulatory complexes. LIM-domain binding protein (LDB) and single-stranded DNA-binding protein (SSDP) function as core scaffolds of transcriptional complexes in animals and plants. Little is known about potential partners and functions for LDB/SSDP complexes in the context of tissue regeneration. In this work, we find that planarian LDB1 and SSDP2 promote tissue regeneration, with a particular function in mediolateral polarity reestablishment. We find that LDB1 and SSDP2 interact with one another and with characterized planarian LIM-HD proteins Arrowhead, Islet1, and Lhx1/5-1. SSDP2 and LDB1 also function with islet1 in polarity reestablishment and with lhx1/5-1 in serotonergic neuron maturation. Finally, we show new roles for LDB1 and SSDP2 in regulating gene expression in the planarian intestine and parenchyma; these functions may be LIM-HD-independent. Together, our work provides insight into LDB/SSDP complexes in a highly regenerative organism. Further, our work provides a strong starting point for identifying and characterizing potential binding partners of LDB1 and SSDP2 and for exploring roles for these proteins in diverse aspects of planarian physiology.
### Competing Interest Statement
The authors have declared no competing interest.
* CLIM
: co-factor of LIM domains
DNA
: deoxyribonucleic acid
dpa
: days post-amputation
ISH
: in situ hybridization
LDB
: LIM domain binding protein
LIM
: LIN-11, Isl-1, and Mec3
LIM-HD
: LIM homeodomain
LHX
: LIM homeobox
LMO
: LIM only
RNA
: ribonucleic acid
SSDP
: single-stranded DNA-binding protein
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关键词
ssdp proteins,transcriptional complexes,planarian ldb,regeneration
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