Cynanotophyllosides E-F, two minor pregnane glycosides from the roots of cultivated Cynanchum otophyllum

Cynanotophyllosides E-F, two new minor pregnane glycosides were isolated from the antidepressant active fraction of cultivated Cynanchum otophyllum, and their structures were determined as 12-O-vanilloyl-deacetylmetaplexigenin 3-O-beta beta-D-glucopyranosyl-(1 -> 4)-beta beta-D-glucopyranosyl-(1 -> 4)-beta beta-D-cymaropyranosyl-(1 -> 4)-beta beta-D-oleandropyranosyl-(1 -> 4)-beta beta-D-digitoxopyranoside, and 12-O-nicotinoyl-deacetylmetaplexigenin 3-O-beta beta-D-glucopyranosyl-(1 -> 4)-beta beta-D-glucopyranosyl-(1 -> 4)-beta beta-D-cymaropyranosyl-(1 -> 4)-beta beta-D-oleandropyranosyl-(1 -> 4)-beta beta-D-cymaropyranoside respectively, with the combination of spectroscopic and chemical analysis.