Electrospun plant protein-based nanofibers loaded with sakacin as a promising bacteriocin source for active packaging against Listeria monocytogenes in quail breast

The main objective of this study was to fabricate nanofibers from zein incorporated with two concentrations of sakacin (9 and 18 AU/mL) with anti-Listeria properties by electrospinning technique. The efficacies of the resulting active nanofibers against L. innocua, in quail breast during 24 days of refrigerated storage (4 +/- 1 degrees C) were evaluated. The minimum inhibitory concentration (MIC) of bacteriocin against L. innocua was approximate 9 AU/mL. Fourier-transform infrared spectra of bacteriocin-loaded nanofibers indicated characteristic peaks of zein and sakacin and that the nanofibers showed an encapsulation efficiency close to 91.5 %. The thermal sta-bility of sakacin increased by electrospinning. Scanning electron microscopy images showed that nanofibers prepared from electrospinning zein/sakacin solutions exhibited smooth and continuous nanofibers with no de-fects with an average diameter between 236 and 275 nm. The presence of sakacin led to decreased contact angle properties. Nanofibers with 18 AU/mL sakacin exhibited the highest zone of inhibition of 226.14 +/- 8.05 mm. The lowest L. innocua (6.1 logs CFU/cm2) growth after 24 days at 4 degrees C were obtained in quail breast wrapped with zein containing 18 AU/mL sakacin. The results demonstrate an outlook for the potential use of zein nanofibers containing sakacin to reduce L. innocua contamination in ready-to-eat (RTE) products.