A monolithic azacalix[4]pyridine column for high-resolution and high-efficiency pressurized capillary electrochromatographic separation

•A highly stable nAC4P@capillary monolithic column was fabricated by super-cross-linking strategy.•The nAC4P@capillary exhibits high separation efficiency and selectivity.•The synergistic effect of the various interactions was confirmed by experiments and non-covalent interaction analysis.