Evaluation of [ 99m Tc]Tc-TTHMP as a SPECT imaging agent for skeletal system

TTHMP (triethylene-tetramine-hexamethylene-phosphonic acid) shows good bone-seeking ability in previous studies. In this research, [ 99m Tc]Tc-TTHMP was prepared with the radiochemical yield of 98% and the possible application of [ 99m Tc]Tc-TTHMP in skeletal system imaging was evaluated. The corresponding TTHMP kits have also been developed. [ 99m Tc]Tc-TTHMP is hydrophilic and stable in saline and serum. The biodistribution data show [ 99m Tc]Tc-TTHMP has a high uptake in the bone and is excreted rapidly through urinary system. Both micro-SPECT/CT imaging in normal mice and SPECT imaging in a normal Beagle dog confirm the results. It is suggested that [ 99m Tc]Tc-TTHMP can be a candidate for skeletal system imaging.