Comparison of two highly sensitive benzodiazepine immunoassay lab developed tests for urine drug testing in clinical specimens.

Kyle Lund,Marlen Menlyadiev,Kyunghoon Lee,Michael J Kelner,Robert L Fitzgerald,Raymond T Suhandynata

Journal of mass spectrometry and advances in the clinical lab（2023）

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摘要

We demonstrate the development and validation of an immunoassay LDT with improved sensitivity for glucuronidated benzodiazepines. This LDT can detect glucuronidated benzodiazepines in clinical urine specimens and is stable for 60 days. Importantly, we were able to validate the immunoassay as an LDT by utilizing an LC-MS/MS LDT.

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关键词

Benzodiazepine,Beta-glucuronidase,High Sensitivity,Immunoassay,LC-MS/MS,Laboratory Developed Tests,VALID Act

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