Enhanced Expression of Xylanase in Aspergillus Niger Enabling a Two-Step Enzymatic Pathway for Extracting Β-Glucan from Oat Bran

Yangyang Li,Cen Li,Sahibzada Muhammad Aqeel,Yachan Wang,Quan Zhang, Jianing Ma,Jingwen Zhou,Jianghua Li,Guocheng Du,Song Liu

BIORESOURCE TECHNOLOGY（2023）

引用 47|浏览17

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摘要

•Multigene integration in A. niger was achieved using a modified CRISPR-Cas9 system.•The xynA activity in A. niger was enhanced 3,650-fold via a combined strategy.•A two-step enzymatic pathway for extracting β-glucan (85.1% purity) was developed.

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关键词

Aspergillus niger,Xylanase,Amylolytic enzyme,Two-step enzymatic pathway,β-Glucan extraction

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