Spatial sterol metabolism unveiled by stimulated Raman imaging

Graphical AbstractHigh-resolution stimulated Raman scattering (SRS) imaging of a genetically engineered model (GEM) enables metabolite imaging in a yeast model and uncovers an unexpected regulatory mechanism of sterol metabolism, providing new insights underpinning the distributional and functional importance of sterol in cells. SRS-GEM demonstrates a promising platform to explore unknown metabolic mechanisms beyond the reach of conventional approaches.