False negative effect of high triglycerides concentration on vitamin d levels: a big data study

Background: Inaccurate test results may be a reason why vitamin D deficiency is seen as a common problem world-wide. Interferences from the sample matrix during testing are the most important factors in measurement errors. In this study, the relationship between triglycerides and total cholesterol levels and vitamin D levels in Turkey was inves-tigated. Methods: The 25-hydroxyvitamin D test results and lipid test results studied in Turkey in 2021 were compared. Data were obtained from the Ministry of Health National Health Database. Simultaneously, 25-hydroxyvitamin D, triglyceri-de, and total cholesterol levels were studied, and 1,135,644 test results were taken as the basis. Results: In the group of patients with total cholesterol levels between 0-10.33 mmol/L, the proportion of patients below 20 & mu;g/L ranged from 56.8% to 61.8%. In the patient group with cholesterol between 10.36-259 mmol/L, the rate of patients with less than 20 & mu;g/L was between 70.8- 100%, while the rate of patients with cholesterol above 100 & mu;g/L was 0%. The mean 25-hydroxyvitamin D level was 20.1 & mu;g/L in the patient group with a total cholesterol level between 0-10.33 mmol/L, and 16 & mu;g/L in the pati-ent group with a cholesterol level above 10.36 mmol/L. The mean 25-hydroxyvitamin D level was 20.11 & mu;g/L in the patient group with triglycerides 0-10.16 mmol/L, and the 25-hydroxyvitamin D level was 12.28 & mu;g/L in the pati-ent group with triglycerides 10.17-113 mmol/L. The pro-portion of patients with vitamin D levels above 100 & mu;g/L was found to be 0% in the group of patients with triglyceri-des above 10.17-113 mmol/L. Conclusions: According to this study, there is a risk of toxi-city when administering vitamin D therapy in patients with high cholesterol and triglycerides levels. This study is the first of this size in the literature. High triglycerides and cho-lesterol levels can cause inaccurate measurement of vita-min D levels, so care should be taken when evaluating these tests.