Supplemental Figures from PanIN Neuroendocrine Cells Promote Tumorigenesis via Neuronal Cross-talk

Figure S1 (Supports Figures 1 and 3) Co-culture of DRG neurons with cell and organoid lines. Figure S2 (Supports Figure 6) Histopathologic techniques for PanIN quantification and tumor identification. Figure S3 (Supports Figure 4) FACS sorting of PanIN neuroendocrine cells and expansion in organoid culture. Figure S4 (Supports Figure 1) Innervation of wild type pancreas and PanIN-free acinar tissue and axon recruitment by PDAC cell lines. Figure S5 (Supports Figure 2) Mouse and human PanINs contain NK1-R+ cells. Figure S6 (Supports Figure 2) Human duodenal enteroendocrine cells express the NK1-R. Figure S7 (Supports Figure 3) PanIN organoids express CgA and neuronal conditioned media increases PanIN organoid proliferation. Figure S8 (Supports Figure 4) Neuroendocrine cells exert trophic influences in vitro. Figure S9 (Supports Figure 6) Quantifications in control and denervated mice. Figure S10 (Supports Figure 6) Quantification of p-Stat3 in vivo. Figure S11 (Supports Figure 6) Quantification of NK1-R+ cells. Table S1. qPCR primer sequences. Video V1 (Supports Figure 1). Video V2 (Supports Figure 4).