Supplementary Methods, Figures 1-6, Tables 1-2 from Vav3-Rac1 Signaling Regulates Prostate Cancer Metastasis with Elevated Vav3 Expression Correlating with Prostate Cancer Progression and Posttreatment Recurrence

PDF file - 339K, Supplemental Materials and Methods Figure S1 Vav3-mediated enhancement of NIH3T3 cell migration and invasion correlates with activation of Rac1. Figure S2 Reduction of Rac1 decreases Vav3 enhanced LNCaP cell migration and invasion. Figure S3 Vav3 phosphorylation in the presence of various growth factors in LNCaP and PC3 cells. Figure S4 Reduced Vav3 expressions decrease level of several MMPs expression, and inhibit ephrinA1-induced migration, invasion, and Rac1 activation. Figure S5 Orthotopic transplantation of PC3 cells into nude mice. Figure S6 Model of receptor tyrosine kinases mediated Vav3-Rac1 signaling in prostate cancer progression and metastasis. Table S1 STR profiles of PC3 parental cell lines and their microscopic bone metastatic lines, PC3-Bone-1 to 3.