Gene Targeting in Rat Embryonic Stem Cells.
Methods in molecular biology (Clifton, N.J.)（2023）
摘要
Rat germline-competent embryonic stem (ES) cell lines have been available since 2008, and rat models with targeted mutations have been successfully generated using ES cell-based genome targeting technology. This chapter will focus on the procedures of gene targeting in rat ES cells.
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关键词
ES cell selection,Gene targeting,Nucleofection,Rat ES cells
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