Supplementary figures and figure legends from Liver Metastasis Is Facilitated by the Adherence of Circulating Tumor Cells to Vascular Fibronectin Deposits

Supplementary Figure S1: CTCs from mCRC patients display and adhesive-like gene expression pattern enriched in focal adhesion components; Supplementary Figure S2: Collagen I and IV are absent in the luminal side of vascular endothelium; Supplementary Figure S3: Liver fibronectin is upregulated in primary tumor bearing mice; Supplementary Figure S4: Luminal fibronectin deposits are present after liver blood perfusion; Supplementary Figure S5: Intravascular fibronectin deposits are present in human livers bearing metastasis from colon cancer; Supplementary Figure S6: Cancer cells display paxillin-GFP aggregates that colocalize with hepatic vascular fibronectin in vivo; Supplementary Figure S7: Validation of talin1 knockdown as a model for focal adhesion-depletion; Supplementary Figure S8: Focal adhesion-impaired cells determine adhesion to different ECM substrates and HUVEC cells; Supplementary Figure S9: Endothelial cell retraction induced by cancer cells; Supplementary Figure S10: Talin1 knockdown does not alter cell viability/proliferation in vitro; Supplementary Figure S11: Intravascular fibronectin deposits are also present in mesenteric blood vessels.