High-resolution confocal and light-sheet imaging of collagen 3D network architecture in very large samples
iScience(2023)
摘要
Summary: Although notoriously difficult, imaging collagen network architecture, a key element affecting tissue mechanical properties, is of paramount importance in developmental and cancer biology. Here, we introduce a simple and robust method of whole-mount collagen staining with the ‘Fast Green’ dye that provides unmatched visualization of collagen 3D network architecture, via confocal or light-sheet microscopy, compatible with solvent-based tissue clearing and immunostaining.
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关键词
Molecular biology,Cell biology,Microstructure
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