Supplemental Information from Ag-Presenting CpG-Activated pDCs Prime Th17 Cells That Induce Tumor Regression

Supplemental Information. Supplemental Materials and Methods Supplemental Fig. 1 : Chimeric mice selectively lacking MHCII expression on pDCs. Supplemental Fig. 2 : Similar T cell proliferation in µMT:WT and µMT pIII+IV-/-:WT chimeric mice. Supplemental Fig. 3 : Selective MHCII abrogation on pDCs in μMT pIII+IV-/-:WT mice does not affect MHCII expression and upregulation in other cell subtypes. Supplemental Fig. 4 : MHCII deficient pDCs exhibit normal TLR responses in vitro. Supplemental Fig. 5 : Vaccination using OVA protein + CpG-B significantly inhibits tumor growth after Ag presentation by pDCs. Supplemental Fig. 6 : Characterisation of in vitro generated Th0 and Th17 OT-II T cells. Supplemental Fig. 7 : Impaired cell migration toward tumor supernatant in mice lacking MHCII on pDCs. Supplemental Fig. 8 : CD8+ T cell depletion in EG7 bearing mice.