Supplementary Figures 1 - 10 from <i>Rb1</i> Haploinsufficiency Promotes Telomere Attrition and Radiation-Induced Genomic Instability

PDF file - 2012K, Cre recombinase mediated deletion of the floxed Rb1 allele (S1); Characterisation of Rb1 expression in the cell lines (S2); Cre transgene expression in bone and explant cultures of primary osteoblasts (S3); Rb1 mRNA levels after transduction with shRB1-expressing lentivirus (S4); In vitro Rb1 excision using lentiviral Cre (S5); Assessment of telomere length in LV-Cre infected cells using genomic PCR (S6); Transduction with the LVRB1 leads to recovery of retinoblastoma protein expression (S7); MDM2 copy number measurement (S8); p53 expression in primary osteoblasts (S9); Formation of acentric fragments (micronuclei) after radiation exposure (S10).