Supplementary Figure 1 from A Universal Strategy for Adoptive Immunotherapy of Cancer through Use of a Novel T-cell Antigen Receptor

PDF file - 67K, Upper. CD28 co-stimulation protects against antigen-induced cell death. Annexin V and 7-AAD staining of T cells (untransduced, BBIR-z and BBIR-28z) following 72 h (grey bars) and 96 h (black bars) co-culture with A1847 at an E:T ratio 1:1, painted with either Bio-IgG1 or Bio-EpCAM antibodies. Apoptosis was quantified as a percentages of apoptotic cells- Annexin V+ and 7AAD+ (means �� SEM; n = 3). Lower. Annexin V/7-AAD assay plots showing T cells after 96 h co-culture with A1847 cell line labeled with biotinylated IgG1 (Bio-IgG1) (top panels) and biotinylated EpCAM specific (Bio-EpCAM) antybodies, at an E:T ratio of 1:1. One representative FACS analysis is shown (n=3).