Supplementary Movie 1 from Real-time <i>In Vivo</i> Molecular Detection of Primary Tumors and Metastases with Ratiometric Activatable Cell-Penetrating Peptides

AVI file - 1355K, Supplementary Movie - Movie of mouse bearing GFP-labeled 8119 primary auricular tumor with a cervical lymph node with partial cancer invasion 2 hours following IV injection of RACPP1. Green color is GFP labeling seen in the primary tumorand on the lateral aspect of the involved lymph node. Images with aqua pseudocolor are generated from the Cy5 emission fluorescence intensity alone superimposed on white light reflectance. Images with multihued pseudocolor varying from dark blue (low ratio) to red (high ratio) are generated from the Cy5/Cy7 ratio at every pixel. Note that region of cancer invasion (GFP positive cells seen as green) in the cervical lymph node correlate with the region of highest Cy5/Cy7 ratio (seen as red pseudocolor).