Supplementary Figure S4 from Radiation Responses of 2D and 3D Glioblastoma Cells: A Novel, 3D-specific Radioprotective Role of VEGF/Akt Signaling through Functional Activation of NHEJ

EGFR and VEGFR2 expression and DNA damage response in vivo. A, Representative immunohistochemistry images for pVEGFR2 of paraffin-embedded orthotopic tumours derived from intracranially injected G7 GSC (left panels) and E2 GSC (right panels), and EGFR immunohistochemistry images in G7 paraffin-embedded (PFE) orthotopic tumours as in (A). B, Following a 5 month period after intracranial injection of E2 cells, mice were treated with 10 Gy and sacrificed at different time points (0, 0.5, 2 and 24 hrs) following radiation treatment. Representative immunofluorescent images of PFE-orthotopic tumours stained for EGFR (red) and Rad51 (green) are depicted. DAPI (blue) was used for nuclear staining. C, Bioluminescence images confirming tumour engraftment CD1 nude mice injected intracranially with U87MGLuc cells on day 6. Images were acquired using a Xenogen IVIS Spectrum and Living Software.