Supplementary Figure S4 from Integration of Downstream Signals of Insulin-like Growth Factor-1 Receptor by Endoplasmic Reticulum Stress for Estrogen-Induced Growth or Apoptosis in Breast Cancer Cells

(A) MCF-7:5C cells were treated with vehicle (0.1% EtOH; con) or E2 (10-9 mol/L) for 72 hours. Cells were harvested in TRIzol. IGF-1Rβ was quantitated by real-time RT-PCR. p<0.001, ** compared with control. (B) MCF-7:5C cells were treated with vehicle (0.1% DMSO; con) or AG1024 (5×10-6 mol/L) for 48 hours. Total-IGF-1Rβ and p-IGF-1Rβ were examined by Western blot.