First Report of Colletotrichum jiangxiense Causing Anthracnose on Chili in Yunnan Province, China

HomePlant DiseaseVol. 107, No. 2First Report of Colletotrichum jiangxiense Causing Anthracnose on Chili in Yunnan Province, China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Colletotrichum jiangxiense Causing Anthracnose on Chili in Yunnan Province, ChinaYuanyuan Zhang, Zhangsheng Zhu, Yongtai Xu, Linjun Yang, Yeting Wang, Changming Chen, Peng Zheng, Si Sun, Erxun Zhou, and Canwei ShuYuanyuan Zhanghttps://orcid.org/0000-0002-5646-5718Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaSearch for more papers by this author, Zhangsheng ZhuCollege of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaSearch for more papers by this author, Yongtai XuGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaSearch for more papers by this author, Linjun YangGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaSearch for more papers by this author, Yeting WangGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaSearch for more papers by this author, Changming ChenCollege of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaSearch for more papers by this author, Peng ZhengCollege of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaSearch for more papers by this author, Si SunCollege of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaSearch for more papers by this author, Erxun Zhou†Corresponding authors: E. Zhou; E-mail Address: [email protected], and C. Shu; E-mail Address: [email protected]https://orcid.org/0000-0003-1193-4661Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaSearch for more papers by this author, and Canwei Shu†Corresponding authors: E. Zhou; E-mail Address: [email protected], and C. Shu; E-mail Address: [email protected]Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaSearch for more papers by this authorAffiliationsAuthors and Affiliations Yuanyuan Zhang1 Zhangsheng Zhu2 Yongtai Xu1 Linjun Yang1 Yeting Wang1 Changming Chen2 Peng Zheng2 Si Sun3 Erxun Zhou1 † Canwei Shu1 † 1Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China 2College of Horticulture, South China Agricultural University, Guangzhou 510642, China 3College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China Published Online:12 Feb 2023https://doi.org/10.1094/PDIS-04-22-0945-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleChili (Capsicum spp.) is one of the most widespread cultivated species of Solanaceae in China (Padilha 2016). C. annuum plays a pivotal role in imparting pungency and flavor to Chinese cuisines and is an important cash crop with high local demand and export potential. In October 2021, typical symptoms of anthracnose were observed in approximately 60% of chili fruit (cv. Lamb chili) in Yunnan province, China. Lesions on the pepper fruit were dark brown, round or irregular, necrotic, dented, and led to soft rot of the flesh, which over time discolored dark brown. During the late stages of development, orange conidial mucus was visible on the lesions. To perform fungal isolation, small tissue pieces from the marginal region of lesions on four symptomatic fruits were superficially disinfested by immersion in a 75% ethanol solution for 10 s and in 5% sodium hypochlorite for 3 min, rinsed in sterile distilled water twice, dried on a clean bench, and placed in a Petri dish (Φ = 9 cm) containing 15 ml of potato dextrose agar (PDA) supplemented with 15 μl of ampicillin (50 mg/ml). Culture plates were incubated at 28°C for 7 days in the dark. After 7 days, a single-spore strain YN296-2 was obtained, with colonies on PDA that were white, with bright orange conidial masses near the inoculum point, and the reverse was white and grayish-green. Conidia (n = 100) were cylindrical, hyaline, aseptate, with rounded ends, and 13.27 to 17.74 × 4.72 to 6.68 μm. Based on morphological features, the isolate was identified as in the C. gloeosporioides species complex (Weir et al. 2012). For molecular identification, genomic DNA of YN296-2 was extracted using the CTAB method (Yan et al. 2018), and partial sequences of actin (ACT), β-tubulin (TUB2), calmodulin (CAL), chitin synthase (CHS-1), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes were separately amplified by PCR and sequenced with ACT-512F/ACT-783R (Carbone and Kohn 1999), T1/Bt2B (Glass and Donaldson 1995), CL1F/CL2AR (1999), CHS-1-79F/CHS-1-354R (Carbone and Kohn 1999), and GDF1/GDR1 (White et al. 1990) primers, respectively. The sequences were deposited in GenBank (accession nos. ACT: ON080554, TUB2: ON080625, CAL: ON080696, CHS-1: ON080767, and GAPDH: ON080768). BLAST analysis of the obtained sequences of the ACT, TUB2, CAL, CHS-1, and GAPDH genes revealed 99.6, 100, 100, 97.2, and 100% identity with those of isolate LPSU20120251 of C. jiangxiense (GenBank accession nos. KX009031, KX009039, KX009040, KX009033, and KX009035, respectively). A phylogenetic tree based on maximum likelihood and including published ACT, TUB2, CAL, CHS-1, and GAPDH data for Colletotrichum species was constructed. The multilocus phylogenetic analysis clearly distinguished YN296-2 as C. jiangxiense, separating it from all other species in the C. gloeosporioides species complex. To conduct Koch’s postulates, 10 healthy chili fruit (cv. Lamb chili) were wounded with a sterile toothpick (3 mm in depth) and 10 μl of conidial suspension (1 × 106 spores/ml) was placed on each wound. Five control fruit were wounded in the same way and treated with 10 μl of sterilized water. All the fruit were kept in an incubator at 28°C with 80% relative humidity for 7 days. All inoculated fruit produced circular, necrotic lesions (Φ = 10 to 20 mm) 7 days after inoculation, and control fruit remained healthy. The fungus was consistently reisolated from the diseased fruit. Previously, C. jiangxiense has been reported as a pathogen on Camellia sinensis and Citrus sinensis in China (Liu et al. 2015) and on avocado in Mexico (Ayvar-Serna et al. 2021). To our knowledge, this is the first report of C. jiangxiense causing anthracnose disease on chili. Therefore, it is necessary to explore the disease mechanism of C. jiangxiense and develop a green prevention and control strategy.The author(s) declare no conflict of interest.References:Ayvar-Serna, S., et al. 2021. Plant Dis. 105:502. https://doi.org/10.1094/PDIS-03-20-0459-PDN Link, ISI, Google ScholarCarbone, I., and Kohn, L. M. 1999. Mycologia 91:553. https://doi.org/10.1080/00275514.1999.12061051 Crossref, ISI, Google ScholarGlass, N. L., and Donaldson, G. C. 1995. Appl. Environ. Microbiol. 61:1323. https://doi.org/10.1128/aem.61.4.1323-1330.1995 Crossref, ISI, Google ScholarLiu, F., et al. 2015. Persoonia 35:63. https://doi.org/10.3767/003158515X687597 Crossref, ISI, Google ScholarPadilha, H. K. M. 2016. 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Google ScholarFunding: This work was supported by the Key-Area Research and Development Program of Guangdong Province 2018B0206001.The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 107, No. 2 February 2023SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Metrics Article History Issue Date: 28 Feb 2023Published: 12 Feb 2023Accepted: 6 Jul 2022 Page: 568 Information© 2023 The American Phytopathological SocietyFundingKey-Area Research and Development Program of Guangdong ProvinceGrant/Award Number: 2018B0206001Keywordsfruitfungipathogen detectionvegetablesThe author(s) declare no conflict of interest.PDF download