Vibrio paucivorans sp. nov. and Vibrio qingdaonensis sp. nov. , two marine bacteria.

Two Gram-stain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacteria, designated strains DBSS07 and ZSDZ65, were isolated from the surface sediments of the Bohai sea and Qingdao coastal seawater, respectively. Phylogenetic analysis based on 16S rRNA genes, multilocus sequence analysis (MLSA), phylogenomic analysis of single-copy gene families and whole-genome data placed DBSS07 and ZSDZ65 within the genus . DBSS07 was most closely related to M22, with 97.51 % sequence similarity, whereas ZSDZ65 was most closely related to R-40492 with 97.58 % sequence similarity. DBSS07 grew with 1-7 % (w/v) NaCl (optimum 3 %), at 16-37 °C (optimum 28 °C) and at pH 6.0-9.0 (optimum pH 7.0); whereas ZSDZ65 grew with 1-5 % (w/v) NaCl (optimum 2 %), at 16-32 °C (optimum 28 °C) and at pH 6.0-9.0 (optimum pH 8.0). Both strains shared the same major fatty acid components (more than 10 % of total fatty acids) of summed feature 3 (Cω7 or/and Cω6), with different proportions. The DNA G+C contents of DBSS07 and ZSDZ65 were 44.7 and 44.3 %, respectively. On the basis of the results of polyphasic analysis, DBSS07 and ZSDZ65 are considered to represent novel species within the genus , for which the names sp. nov. (type strain, DBSS07 = KCTC 82896= MCCC 1K06284) and sp. nov. (type strain, ZSDZ65 = KCTC 82893 = MCCC 1K06289) are proposed, respectively.