Improvement Effect of Membrane-Free Stem Cell Extract on Atopic Dermatitis in NC/Nga Mice

Membrane-free stem cell extract (MFSCE) derived from adipose tissue has been reported to have anti-inflammatory activity. In the present study, we investigated the effects of MFSCE on atopic dermatitis (AD)-like skin inflammation using house-dust-mite-sensitized NC/Nga mice. Topical application of MFSCE significantly ameliorated AD-like clinical symptoms including erythema, dry skin, edema, excoriation, erosion, lichenification, and scratching. In addition, the levels of serum immunoglobulin E and inflammatory cytokines were decreased by MFSCE treatment. Furthermore, treatment with MFSCE inhibited the increase in epidermal thickness, infiltration of mast cells, expression of interleukin (IL)-4, IL-10, interferon-γ, tumor necrosis factor-α, thymus, and activation-regulated chemokines in the dorsal skin of NC/Nga mice. In conclusion, MFSCE effectively suppressed AD-like manifestations preclinically, systemically, and topically. Our study suggests that MFSCE may be a useful natural product for AD therapeutic strategies.