Emergence and Inter- and Intrahost Evolution of Pandrug-Resistant Klebsiella pneumoniae Coharboring tmexCD1-toprJ1, bla NDM-1 , and bla KPC-2 .

Klebsiella pneumoniae is capable of acquiring various exogenous genetic elements and subsequently conferring high antimicrobial resistance. Recently, a plasmid-mediated RND family multidrug efflux pump gene cluster, , was discovered in K. pneumoniae. In this study, we analyzed tigecycline-resistant K. pneumoniae isolates from patients from surveillance from 2017 to 2021. In addition to phenotype detection, including growth curves, plasmid transferability and stability, hypermucoviscosity, biofilm formation, and serum survival, by whole-genome sequencing, we analyzed the phylogenetic relationships of the isolates harboring and discovered the composition of plasmids carrying . In total, we discovered that 12 tigecycline-resistant isolates from 5 patients possessed , designated sequence type 22 (ST22) and ST3691. An ST11 isolate harbored a partial , and a complete ( was lost) was tigecycline sensitive. All the ST22 tigecycline-resistant isolates coharbored , , and . was encoded by a novel IncU plasmid in ST22 and an IncFIB/HI1B plasmid in ST3691, which presented differences in mobility and stability. Interestingly, isolates from the same patients presented heteroresistance to tigecycline, not only among isolates from different specimens but also those from the same sample, which might be attributed to the differential expression of due to the dynamic genetic heterogeneity caused by relocating close to the replication origin of plasmid. Here, we reported the emergence of K. pneumoniae isolates coharboring , , and . The results highlight the impact of genetic heterogeneity of -carrying elements on the variation of tigecycline resistance, which might have notable influences on antimicrobial treatment. Pandrug-resistant (PDR) Klebsiella pneumoniae poses a great challenge to public health, and tigecycline is an essential choice for antimicrobial treatment. In this study, we reported the emergence of PDR K. pneumoniae coharboring , , and , which belongs to ST22 and ST3691. By whole-genome analysis, we reconstructed the evolutionary map of the ST22 ancestor to become the PDR superbug by acquiring multiple genetic elements encoding or . Importantly, the genetic contexts of among the ST22 isolates are different and present with various mobilities and stabilities. Furthermore, we also discovered the heterogeneity of tigecycline resistance during long-term infection of ST22, which might be attributed to the differential expression of due to the dynamic genetic heterogeneity caused by relocating close to the replication origin of plasmid. This study tracks the inter- and intrahost microevolution of the superbug PDR K. pneumoniae and highlights the importance of timely monitoring of the variation of pathogens during antimicrobial treatment.