Expression Analysis of M6a-Related Genes in Various Tissues of Meishan Pigs at Different Developmental Stages
REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE(2023)
摘要
To characterize the N6-methyladenosine (m 6 A)-related gene expression profiles in various tissues of Meishan pigs at different stages, m 6 A modificationrelated genes (METTL3, METTL14, METTL16, WTAP, RBM15, and FTO) were detected from newborn to physical maturity of Meishan pigs at eight important developmental stages (1, 7, 14, 21, 28, 35, 134, and 158 days old).The expression of m 6 A-related genes was tissue-specific.Furthermore, the level of METTL3 messenger RNA (mRNA) was higher on day 35 than in other stages in most tissues, and the expression of METTL14 increased after day 35, and FTO exhibited a peak on day 14 in muscle, intestine, lymph nodes, thymus, and kidney.This study provided a reference for an in-depth study of the expression patterns of m 6 A modification-related genes in Meishan pigs.
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关键词
FTO,METTL3,tissue expression profile,weaned piglets
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