Synthesis and Cytotoxic Evaluation of New Fluoro and Trifluoromethyl Substituents Containing Chromeno[2,3-d]pyrimidines

In this research, microwave-assisted "one-pot" multicomponent reactions have been employed for the synthesis of new chromeno[2,3-d]pyrimidines containing fluorine atoms. The biological significance of the obtained compounds was highlighted by evaluating in vitro for cytotoxic activity against four human cancer cell lines (HepG2, hepatoma carcinoma; MCF7, breast cancer; A549, non-small lung cancer; KB, epidermoid carcinoma). Interestingly, two compounds revealed significant inhibitory activity against A549 cell line, and showed low toxicity to human embryonic kidney (Hek-293) cell line. The synthetic efficacy and cytotoxic potency have offered an opportunity to further development of chromeno[2,3-d]pyrimidines containing fluorine atoms as potential anticancer agents.