Indirect functional effects of neighbors on food web compartments could not overcome density-dependent limited growth of a grazing minnow

Ecosystems include diverse taxonomic and functional groups of animals with variable levels of niche overlap that can lead to direct and indirect interactions with positive or negative outcomes. Species feeding at low trophic positions might be sensitive to interactions with co-occurring functional groups (neighbors) that influence their food resources through various processes, such as indirect nutrient subsidies and trophic cascades. Employing a target-neighbor experimental design using large, outdoor mesocosms, we tested whether adding neighbors with different functional traits affected food web compartments relative to a control group that had grazing minnows, Campostoma anomalum (Central Stoneroller). Additionally, we tested whether growth and body condition of Central Stoneroller was positively influenced through interactions with each neighbor treatment: additional stonerollers, benthic-insectivorous fish Etheostoma spectabile (Orangethroat Darter), terrestrial insectivorous fish Lepomis megalotis (Longear Sunfish), or filter-feeding mussels (two-species assemblage) that are predicted to increase primary production through nutrient enrichment, a trophic cascade or both. Over the 29-day experi-ment, the presence of neighbors had generally negative or highly variable effects on food web compartments. Stoneroller growth was not evident during the experiment and body condition generally declined but was not influenced by neighbor treatments. Our results show that adding neighboring functional group did not reduce density-dependent growth of grazing fishes via indirect food web interactions over short temporal scales. This study suggested that indirect interactions among species and their combined influence on ecosystem properties were weak under experimental conditions representing typical prairie stream communities.