miR-141-Modified Bone Marrow Mesenchymal Stem Cells (BMSCs) Inhibits the Progression of Severe Acute Pancreatitis

The therapeutic effects of bone marrow mesenchymal stem cells (BMSCs) on severe acute pan-creatitis (SAP) and miRNAs are currently the research hotspots. This study intends to explore the potential impact of miR-141-modified BMSCs on SAP. After establishment of rat model of SAP, the animals were grouped into control group, model group, BMSCs group, miR-141 group, positive con-trol group, and PI3K/mTOR signaling agonist group (agonist group) followed by analysis of miR-141 expression by RT-qPCR and the expression of serum amylase, IL-6, TNF-a, TAP, PI3K, mTOR, and LC3-II by Western blot and ELISA. miR-141 was significantly up-regulated in the miR-141-modified BMSCs group (p > 0.05). The contents of serum amylase, IL-6, TNF-a, and TAP was increased in SAP rats and decreased after BMSC treatment (p > 0.05). The increased autophagy flux in the rats with SAT was reduced upon treatment with BMSCs and autophagy flux was decreased in miR-141 group but increased in positive control group. The model and positive control group presented highest expression of LC3-II, p-PI3K and p-mTOR, followed by BMSCs group and miR-141 group (p < 0.05). In conclusion, miR-141-modified BMSCs decrease the phosphorylation of PI3K and IP: 8.46.247.10 On: Thu, 09 Feb 2023 08:18:00 mTOR to inhibit PI3K/mTOR signaling activity and downregulate LC3-II protein to inhibit autophagy, Copyright: American Scientific Publishers thereby ameliorating the development of SAP,indicating that miR-141 might be a therapeutic target Delivered by Ingenta for SAP.