Supplementary Methods, Figures 1 - 9, Table 1 from Small GTPase RhoE/Rnd3 Is a Critical Regulator of Notch1 Signaling

PDF file - 557KB, Supplementary Methods Suppl. Table 1. Primers used for real time and conventional RT-PCR . Suppl. Figure S1. RhoE expression is down-regulated in skin SCC tumors. Suppl. Figure S2. Notch activation does not induce expression of members of the Rho GTPase family of proteins. Suppl. Figure S3. Endogenous activation of Notch signaling induces the expression of Hey2. Suppl. Figure S4. Depletion of RhoE inhibits downstream Notch target genes. Suppl. Figure S5. Real-time RT-PCR analysis of mRNA isolated from primary keratinocytes. Suppl. Figure S6. Levels of RhoE and Keratin1 are increased during keratinocyte differentiation. Suppl. Figure S7. RhoE ablation suppresses growth and commitment to differentiation. Suppl. Figure S8. Negative control for RhoE and Notch1 staining in SCC4 cells. Suppl. Figure S9. Depletion of RhoE abrogates the binding between NIIC and importin Beta1.