Supplementary Figure 3 from Inducible Nitric Oxide Synthase Drives mTOR Pathway Activation and Proliferation of Human Melanoma by Reversible Nitrosylation of TSC2

PDF file - 77K, A375 cells were cultured with the indicated concentrations of the NO donor S-nitroso-acetyl-penicillamine (SNAP), or the NO scavenger PTIO for 16 Hours prior to harvest and preparation of protein lysates.. Biotin switch assay was performed as described in Figure 6, and immunoblotting for BCL-2 and AKT performed after streptavidin pull-down to determine nitrosylated levels of these proteins.