Figure S1-9 from Multiregional Sequencing Reveals Genomic Alterations and Clonal Dynamics in Primary Malignant Melanoma of the Esophagus

Figure S1: Geographic distribution of multiregional samples obtained from the dissection of esophagectomy PMME specimens. Figure S2: Hematoxylin and eosin-stained (H&E) and immunostained sections of spatial samples in PMME. Figure S3: Comparison of variant allele frequency between members in each tumor clade. Figure S4: BAF (B allele frequencies) profiles and LogR values across the genome of PMME. Figure S5: CCF distribution in each spatial samples. Figure S6: Metastastic subclones containing pathogenic mutations of ARHGAP26 and PIK3CA, and arm level chromosome deletion in Chr13 experienced subclonal expansion in metastasis sites. Figure S7: Composite copy number profiles for primary melanoma (n=78) and metastasis melanoma (n=283) with gains in red and losses in blue. Figure S8: Venn diagram of nonsynonymous mutations between ME1, N3 and primary tumor clone. Figure S9: Determine threshold for detecting copy number alterations in ME1 and N3.