Supplementary Methods from miR-205 Targets PTEN and PHLPP2 to Augment AKT Signaling and Drive Malignant Phenotypes in Non–Small Cell Lung Cancer

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PDF file, 107K, Includes plasmids, viral production, transfection methods, and luciferase, qPCR, ELISA, MTT, Colony formation, Anchorage-independent growth, RIP, ChIP, HUVEC tube formation, CAM, and IHC assays.

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