Figure S1 from Tissue Stiffness and Hypoxia Modulate the Integrin-Linked Kinase ILK to Control Breast Cancer Stem-like Cells

Stiff and hypoxic microenvironments regulate beta1-integrin, ILK, and CSC markers in human breast cancer cells. (A) Phase contrast images of MDA-MB-231 cells cultured on soft or stiff substrata under normoxia or hypoxia. Transcript levels of (B) ITGB1 and (C) ILK in MDA-MB-231 cells cultured on soft or stiff substrata under normoxia or hypoxia. (D) Immunoblotting analysis for beta1-integrin, ILK, and GAPDH in MDA-MB-231 cells cultured on soft or stiff substrata under normoxia or hypoxia. Transcript levels of (E) CD44, (F) Nanog, and (G) CD49f in MDA-MB-231 cells cultured on soft or stiff substrata under normoxia or hypoxia. (H) Immunoblotting analysis for CD44 and Nanog in MDA-MB-231 cells cultured on soft or stiff substrata under normoxia or hypoxia. Histograms of flow cytometry analysis of (I) CD44 and (J) Nanog expression in 4T1 cells cultured on soft or stiff substrata under normoxia or hypoxia. (K) Dot plots of flow cytometry analysis of ALDH activity in 4T1 cells cultured on soft or stiff substrata under normoxia or hypoxia. Scale bars, 50 µm. Shown are mean (plus minus) s.e.m. *, P<0.05; **, P<0.01; ***, P<0.001.