Figure S2 from Tissue Stiffness and Hypoxia Modulate the Integrin-Linked Kinase ILK to Control Breast Cancer Stem-like Cells

ILK is required for CSC development in human breast cancer cells. (A) qRT-PCR and immunoblotting analysis for ILK in MDA-MB-231cells stably expressing shRNA against ILK (shILK) or scrambled sequence control (shctrl). Transcript levels of (B) ITGB1, (C) ILK, (D) CD44, (E) Nanog, and (F) CD49f in MDA-MB-231-shctrl or MDA-MB-231-shILK cells cultured on soft or stiff substrata under normoxia or hypoxia. Phase contrast images and quantification of (G) secondary mammospheres and (H) colonies formed in soft agar by MDA-MB-231-shctrl and MDA-MB-231-shILK cells. Scale bars, 50 µm. Shown are mean (plus minus) s.e.m. *, P<0.05; **, P<0.01; ***, P<0.001.