Supplementary Figure 6 from TMEM16A Induces MAPK and Contributes Directly to Tumorigenesis and Cancer Progression

PDF file - 101K, Cyclin D1 expression is reduced in the proliferating tissues derived from Tmem16a -/- mice when compared to wild-type littermates. Tissues harvested from Tmem16a -/- and wild-type mice were subjected to immunohistochemical staining for cyclin-D1. The proliferating mesoderm demonstrated intense nuclear staining of cyclin-D1 in wild-type mice, but not in knock-out littermates (A). Levels of cyclinD1 mRNA are correlated with TMEM16A expression in tissues derived from TMEM16A (-/-), (+/-) and (+/+) mice (B). TMEM16A overexpression did not induce activation of ERK5, another MAPK that can contribute to proliferation (C).