Figure S4 from the Small GTPase ARF6 Activates PI3K in Melanoma to Induce a Prometastatic State

Figure S4. Primary tumor incidence, growth, survival and metastasis in Ptenf/f vs. Arf6Q67L mice. (a) Increased tumor incidence (% = # of mice that developed a tumor / # TVA positive, injected mice) in Ptenf/f vs. Arf6Q67L cohorts. Fisher's exact test, two-tailed, α <0.05. (b) Primary tumor onset, unpaired t test with Welch's correction. (c) Primary tumor growth in Ptenf/f vs. Arf6Q67L mice upon tumor onset. Bars = SD. Two-way ANOVA, a=0.05. (d) Overall survival upon tumor onset, log-rank (Mantel-Cox) test. (e) Comparison of spontaneous metastatic volume in each mouse cohort (see also schematic, Figure 1g). ANOVA (p < 0.0001) with Kruskal-Wallis multiple comparisons test (p values shown). ns=not significant. Identical data from the Control and Arf6Q67L cohorts are also shown in Figure 1c with a slightly different Y axis spacing.