Figure S4 from Anti-CD137 Suppresses Tumor Growth by Blocking Reverse Signaling by CD137 Ligand

Bone marrow cells of WT or CD137-/- mice were cultured in plates coated with human IgG1 or CD137-Fc¬ in the presence of murine Flt3L for 9 d. IFN-gamma and GM-CSF were added, and cells were cultured for an additional 2 d to polarize differentiated DCs toward CD103+ subsets. CD103+ DCs (middle panel) were gated from CD11c+F4/80- cells (upper panel). Macrophages are categorized as F4/80+CD11b+CD11c+ or F4/80+CD11b+CD11c- cells (upper and lower panels).