Supplementary Figure S1 from TWIST1-Induced miR-424 Reversibly Drives Mesenchymal Programming while Inhibiting Tumor Initiation

Supplementary Figure S1 SNAI1 induces mesenchymal genes prior to repression of epithelial genes, concomitant with miR-424 upregulation. (A) Phase contrast images of HMLE Empty Vector and HMLE SNAI1-inducible cells after 4 and 12 days of SNAI1 induction. Bar-100 μm. Epithelial and mesenchymal markers, by qPCR, after (B) 4 and (C) 12 days of SNAI1 induction, two-tailed unpaired t-test. (D) Mature and (E) primary miR-424 levels, by qPCR, after 4 days of SNAI1 induction, two-way ANOVA, Bonferroni multiple comparison post-test. SEM shown, representative images/graphs of n{greater than or equal to}3. #p<0.1, *p<0.05, **p<0.01, ***p<0.001.