Supplementary Tables S1-S2, Figures S1-S9, and Methods from Crosstalk between KIT and FGFR3 Promotes Gastrointestinal Stromal Tumor Cell Growth and Drug Resistance

Supplementary Tables S1-S2, Figures S1-S9, and Methods Table S1: Raw siRNA screening results Figure S1: Response of GIST cell lines to the KIT inhibitor, imatinib Figure S2: Testing of individual FGFR3 siRNA duplexes for efficacy of reducing GIST T1 and GIST 10R cell viability Figure S3: Potential degradation product of FGFR3 upon inhibition with a small-molecule inhibitor Figure S4: FGF1 restores KIT phosphorylation and rescues GIST cells from imatinib inhibition Figure S5: SCF rescues GIST cells from FGFR inhibition Figure S6: FGFR expression levels after siRNA knockdown Figure S7: Combination of B-RAF inhibitor with imatinib is synergistic for GIST 10R cells Figure S8: FGF2 expression levels after siRNA knockdown Figure S9: Illustration of patient tissue analysis using the Aperio ScanScope CS Slide Scanner Supplemental Materials and Methods