Supplementary materials and methods from miRNA-520f Reverses Epithelial-to-Mesenchymal Transition by Targeting ADAM9 and TGFBR2

The supplementary materials and methods file contains a more detailed description of methods (cell culture, real-time and stem loop RT-PCR, Western blot analysis, and 3'-UTR reporter assays) that are described in the main text in brief. Methods that have not been described in the main text, because the data are not shown or only provided in the supplementary figures (Sanger DNA sequence analysis, phase-contrast imaging, and immunocytochemisty) are also described here.