Data Supplement from AR-Regulated TWEAK-FN14 Pathway Promotes Prostate Cancer Bone Metastasis

Supplemental Figure 1.(A) In vitro growth assay for DU145/RasB1 and DU145/RasB1Fn14shRNA cell lines. (B) Average body weight of mice bearing DU145/RasB1 tumors (n= 9mice) or DU145/RasB1Fn14shRNA tumors over time after tumor cell inoculation (shRNA1, n=10 mice; shRNA2, n=10 mice). * p<0.05 vs EV. (C) Average BLI signal in brain metastasis in mice bearing DU145/RasB1/EV, or DU145/RasB1Fn14shRNA tumor at week 4. *** p<0.001 vs control (D) In vitro growth rate of PC3/EV cells and PC3/Fn14shRNA cell lines. (E) Average BLI signal in bone metastasis per mouse in mice bearing PC3/EV tumors or PC3/Fn14shRNA tumors at week 4. Numbers represent mice with bone metastasis over total mice tested. *p<0.05, **p<0.01 vs EV. (F) Survival curve of mice bearing PC3/EV or two PC3/Fn14shRNA cell lines.**p<0.01 vs EV.