Supplementary Figure S1 from Enhanced Histone Deacetylase Activity in Malignant Melanoma Provokes RAD51 and FANCD2-Triggered Drug Resistance

HDAC1, 2 and 3 expression in melanomas. (A) Microarray expression data of HDAC1, HDAC2 and HDAC3 from 45 primary melanoma,18 benign skin nevi, and 7 normal skin tissue specimens. (B) Western blot analysis of HDAC1, HDAC2, HDAC3 and HDAC8 protein levels in the melanoma cell lines D05, G361, SK-Mel187, Mel537, A2058 and A375 compared to the normal (non-cancerous) cells PBLCs, VH10tert and Hema-LP. β-actin served as loading control. Relative expression (R.E.) compared to the lowest protein level is indicated. (C) Relative HDAC protein level in melanoma cells (D05, G361, Mel187, Mel537, A2058 and A375) compared to non-cancerous (PBLCs, VH10tert and Hema-LP) cells as determined by Western blot analysis. (D) Western blot analysis of acetylated-H3 in Hema-LP, PBLCs and VH10tert cells following VPA treatment (1mM for 168 h). H3 was used as loading control. **p<0.005, ***p<0.0001.