Supplementary Figures S1-S10, Materials and Methods from Mechano-Signal Transduction in Mesenchymal Stem Cells Induces Prosaposin Secretion to Drive the Proliferation of Breast Cancer Cells

This file contains supplementary figures S1-S10 and supplementary materials and methods. Figure S1 shows experimental design of mesenchymal stem cells in this article. Figure S2 shows differentiation of transgenic mesenchymal stem cells. Figure S3 shows differentiation and gel contraction of mesenchymal stem cells. Figure S4 shows YAP activity in mesenchymal stem cells on a stiff matrix. Figure S5 shows expression and activation of YAP-related signaling molecules in mesenchymal stem cells. Figure S6 shows contribution of mesenchymal stem cells to tumor progression. Figure S7 shows prosaposin detection in mammary tumors. Figure S8 shows the role of prosaposin for ERK and caspase 3. Figure S9 shows contribution of prosaposin to late tumor growth and progression. Figure S10 shows the role of stiff matrix for mesenchymal stem cells from B6 mice.