Supplementary Figure 2 from FOXC2 Expression Links Epithelial–Mesenchymal Transition and Stem Cell Properties in Breast Cancer

Supplementary Figure 2 PDF File - 600K, Ectopic expression of FOXC2 induces EMT in transformed human mammary epithelial cells (HMLER). (a) Quantification of EMT marker mRNA expression by quantitative real-time PCR analysis. Data is represented as the expression of EMT markers in HMLER-FOXC2 (FOXC2) cells relative to HMLER-Vector control cells (Vector). GAPDH was used as an internal normalization control. Columns indicate the mean (n = 3); error bars indicate SEM. (b) Western blot analysis of EMT marker protein expression upon FOXC2 overexpression in HMLER cells. Actin was used as a loading control. (c) Phase contrast and immunofluroescence images of HMLER-Vector and HMLER-FOXC2 cells. Overlayed images are shown for respective EMT markers (red) and DAPI nuclear stain (blue). Scale bar indicates 50 �m