Supplementary Figure 1 from DDX31 Regulates the p53-HDM2 Pathway and rRNA Gene Transcription through Its Interaction with NPM1 in Renal Cell Carcinomas

PDF file - 1.7MB, S1 (A) western blot analysis of DDX31 transfected HEK293 cells (left panel) and endogenous DDX31 in Caki-1 cells (right panel) using purified anti-DDX31 polyclonal antibody. (B)NPM1 protein expression in RCC and HK-2 cells. (C) NPM1 expression in clinical RCC cases by quantitative RT-PCR. (D) immunohistochemical analysis of NPM1 in ccRCC tissue sections. Representative images of ccRCC tissue (776T) and normal kidney tissue (776N). Scale bar shows 50 mum