Supplementary Figure S1 from Regulation of FANCD2 by the Mtor Pathway Contributes to the Resistance of Cancer Cells to DNA Double-Strand Breaks

Supplementary Figure S1 - PDF file 2155K, The mTOR Pathway Regulates FANCD2. A, Rh30 cells were exposed to AZD8055 (2 M) for the times indicated and total proteins were extracted for immunoblotting to detect FANCD2. B, Rh30 cells were transfected with vector, AKT1-Myc plasmid encoding wild type AKT1. 48 hr later, total proteins were extracted for detection of FANCD2, GSK3, pGSK3-S9, AKT and pAKT-S473. Myc antibody was used to show the expression of AKT1 wt with Myc tag. C, Rh30 cells were transfected with vector, Myr-AKT1 plasmid encoding constitutively active AKT1. 48 hr later, total proteins were extracted for detection of FANCD2, GSK3, pGSK3-S9, AKT and pAKT-S473. D, Rh30 cells were treated with rapamycin (100 ng/mL), AZD8055 (2 M) or MK2206 (10 M) for 16 hr. Total proteins were extracted for immunoblotting of FANCD2, S6K1, pS6K1-T389, AKT and pAKT-S473. GAPDH and -Actin served as loading control