Integrated Approaches for the Separation and Purification of Recombinant HPV16 E6 Protein from Escherichia Coli Crude Extracts

•His6-MBP-E6 is isolated and recovered from E. coli lysates by sonication/ice cycles.•MBPTrap column allows an effective capture step for His6-MBP-E6.•SEC separates pre-purified His6-MBP-E6 from MBP proteins.•An imidazole gradient allows His6-MBP-E6 purification from MBP by IMAC.•His6-MBP-E6 secondary structure is maintained during isolation and purification.